Loading Events

« All Events

  • This event has passed.

BIFI TALK – Javier García-Nafría

October 4 @ 1:00 pm - 2:00 pm

Título: Cryo-electron microscopy: moving beyond X-ray crystallography for membrane receptors and drug discovery

Ponente: Javier García-Nafría (LMB/MRC, Cambridge, UK-University of Zaragoza)

Día y hora:  4 de octubre a las 13:00 h

Lugar: SALA DE CONFERENCIAS, EDIFICIO I+D+i, CAMPUS RIO EBRO

Resumen:

Cryo-electron microscopy (cryo-EM) has undergone major advances during the last years, with special impact in structural biology of membrane proteins. I will review the advances in cryo-EM technology that made the ¨resolution revolution¨ possible, and then show its application to understand G-protein coupled receptors (GPCRs). GPCRs are the largest family of membrane proteins in the human body and are a target for ~35% of the currently approved FDA drugs. GPCRs sense a variety of molecules and transduce the signal to the intracellular milieu by coupling to heterotrimeric G-proteins. We used cryo-EM to obtain high-resolution structural information on GPCR-G protein complexes, starting to tackle one of the major questions in the field – the GPCR-G protein selectivity code. I will then analyse the state-of-the-art in using cryo-EM for structure-based drug discovery of membrane proteins.

References:

  1. Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein. García-Nafría J.*, Lee Y.*, Bai X., Carpenter B. & Tate CG. 2018. Elife. May 4;7. pii: e35946.
  1. Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go. García-Nafría J., Nehme R., Edwards P., Tate CG. 2018. Nature 558 (7711). 620-62
  1. Cryo-EM structures of GPCRs coupled to Gs, Gi and Go.  García-Nafría J. and Tate CG. Molecular and Cellular Endocrinology. 2019, In press.
  1. Cryo-Electron Microscopy: Moving Beyond X-ray Crystal Structures for Drug receptors and Drug development. García-Nafría J. and Tate CG. 2019. Annual Reviews in Pharmacology and Toxicology. 2020. In press (2019 Jul 26. doi: 10.1146.)

 

Details

Date:
October 4
Time:
1:00 pm - 2:00 pm
Event Category:

Venue

Edificio Institutos I+D
Mariano Esquillor, 50018 Zaragoza
Zaragoza, Zaragoza 50018 España
+ Google Map
Phone:
+34 976762989
Website:
http://bifi.es
Contact Us

We're not around right now. But you can send us an email and we'll get back to you, asap.

Not readable? Change text. captcha txt

Start typing and press Enter to search